rabbit anti mef2  (Developmental Studies Hybridoma Bank)

Journal: iScience

Article Title: The Drosophila Him gene is essential for adult muscle function and muscle stem cell maintenance

doi: 10.1016/j.isci.2026.114670

Figure Lengend Snippet: HimGFP design and expression in leg disc myoblasts and adult MuSCs (A) Schematic of the HimGFP “minigene” comprising 3.8 kb upstream genomic sequence, mGFP fused to the Him coding sequence via an -SSSS- linker, followed by the Him 3′ UTR. (B) Larval T2 leg discs labeled with anti-Mef2 and anti-GFP show co-expression of HimGFP and Mef2 (examples arrowed) in the associated myoblasts ( n = 4). Scale bars, 100 μm. (C) Sagittal thoracic section showing an adult MuSC (arrows) on the periphery of a DLM fiber (boxed area shown at higher magnification). The MuSC expresses HimGFP and canonical marker Zfh1, and stains with nuclear marker Hoechst ( n = 5). Scale bars, 100 μm. (D) Close-up of a sagittal thoracic section showing adult MuSCs (arrowed) on the periphery of a DLM fiber that express both HimGFP and Mef2 ( n = 3). Scale bars, 20 μm.

Article Snippet: Rabbit anti-Mef2 (1:1,000) , DSHB , Mef2.

Techniques: Expressing, Sequencing, Labeling, Marker

Journal: bioRxiv

Article Title: Fasting reverses PLN R14del-mediated cardiomyopathy through lysosomal reactivation

doi: 10.64898/2026.03.24.713684

Figure Lengend Snippet: A. Volcano plot showing all significantly (p-value < 0.05) downregulated (n = 201) and upregulated (n = 433) genes in wild type (WT) and plna R14del zebrafish hearts with a log₂(fold change) higher than +/− 0.7. Each dot represents one gene; blue corresponds to downregulated genes and green to upregulated genes. The p-value cut off corresponds to 1.30 on the -log 10 axis. B. Fold enrichment of downregulated and upregulated GO terms identified using the DAVID online GO analysis tool, when comparing WT and plna R14del zebrafish hearts in a pseudo-bulk dataset. C. Representative images of immunofluorescent staining against DAPI, Pln and Lamp1 in one year old WT and plna R14del zebrafish. Data shown are representative for n = 8 WT and n = 8 plna R14del zebrafish hearts. Scale bar = 5 μm. White arrowheads indicate Pln and Lamp1 co-localization. D. Quantification of number of Lamp1 foci divided by cardiomyocyte (CM) area. Statistics were performed by two-tailed unpaired t-test. Data obtained from n = 8 WT and n = 8 plna R14del zebrafish hearts. E. Quantification of Pln puncta co-localized with Lamp1 foci. Data obtained from n = 8 WT and n = 8 plna R14del zebrafish hearts. F. Representative image of immunofluorescent staining against DAPI, PLN and LAMP1 in left ventricle (LV) from patients with end-stage cardiomyopathy carrying the PLN R14del variant. Dashed box high lights the zoomed in area. Data shown are representative for n = 4 individuals. Scale bars = 10 μm. White arrowheads indicate PLN and LAMP1 co-localization. G. Representative images of immunoelectron microscopy against Lamp1 (10 nm gold) in one year old WT and plna R14del zebrafish hearts. * Indicate endo-lysosomal compartments. Data shown are representative for n = 3 individuals. Scale bar = 200 nm. H. Representative images of DQ red BSA BSA ex vivo assay in one year old WT and plna R14del hearts. Dots represent functional lysosomes. Data shown are representative for n = 4 WT and n = 3 plna R14del zebrafish hearts. Scale bar = 10 μm. I. Quantification of number of DQ red BSA foci per 5000 μm 2 . Data points represent an individual measurement per image; multiple points originate from one individual. Data obtained from n = 4 WT and n = 3 plna R14del zebrafish hearts. Error bars indicate mean ± s.d. Statistics were performed by two-tailed unpaired t-test. J. Representative images of DQ red BSA ex vivo assay in WT cardiac slices treated with medium or thapsigargin (100 μM). Dots represent functional lysosomes. Data shown are representative for n = 3 per group. Scale bar = 10 μm. K. Quantification of number of DQ red BSA foci per 5000 μm 2 . Data points represent an individual measurement per image; multiple points originate from one individual. Data obtained from n = 3 zebrafish hearts per group. Error bars indicate mean ± s.d. Statistics were performed by two-tailed unpaired t-test.

Article Snippet: Sections were then blocked for 5 min with 1% BSA-c/VG and incubated for 1 hour at room temperature with a primary antibody against Lamp1 (1:10, Boster Biological Technology, DZ01398-1).

Techniques: Staining, Two Tailed Test, Variant Assay, Immuno-Electron Microscopy, Ex Vivo, Functional Assay

Journal: bioRxiv

Article Title: Fasting reverses PLN R14del-mediated cardiomyopathy through lysosomal reactivation

doi: 10.64898/2026.03.24.713684

Figure Lengend Snippet: A. Representative images of 565 NHS ester general protein stain in one year old wild type (WT) and plna R14del expanded zebrafish hearts. * Indicates relaxed cardiomyocytes; ∼ indicates contracted cardiomyocytes. Data shown are representative for n = 3 WT and n = 3 plna R14del zebrafish hearts. Scale bar upper panel = 5 μm; scale bar lower panel = 2 μm; scale bars are corrected for pre-expansion dimensions. B. Representative images of immunofluorescent staining against Pln and Ca v -Pan in one year old WT and plna R14del zebrafish. Data shown are representative for n = 4 WT and n = 8 plna R14del zebrafish hearts. Scale bar = 5 μm. Line indicates measurement spot for panel C. C. Representative line plots showing Pln (magenta) and Ca v -Pan (grey) normalized intensity in WT and plna R14del cardiomyocytes. D. Representative images of immunofluorescent staining against Mef2 in one year old WT and plna R14del zebrafish. Data shown are representative for n = 6 WT and n = 7 plna R14del zebrafish hearts. Scale bar = 5 μm. E. Quantification cardiomyocyte longitudinal nucleus length in μm based on the Mef2 staining in one year old WT and plna R14del zebrafish hearts. Statistics were performed by two-tailed unpaired t-test. Data obtained from n = 6 WT and n = 7 plna R14del zebrafish hearts. F. Representative image of immunofluorescent staining against DAPI and PLN in left ventricular (LV) tissue obtained from patients with end-stage cardiomyopathy carrying the PLN R14del variant. Data shown are representative for n = 2 individuals. Scale bar = 5 μm.

Article Snippet: The following primary antibodies were used across all samples: PLN (1:100, Invitrogen, MA3-922), γ-TUB (1:400, Sigma-Aldrich, T6557), LAMP1 (1:50, Abcam, ab24170), α-TUB (1:200, Sigma-Aldrich, T5168), Ca v -Pan (1:200, Alomone labs, ACC-004), Mef2 (1:500, Boster Biological Technology, DZ01398-1), GRP78 (1:200, Proteintech, 11587-1-AP) and p62 (SQSTM1) (1:500, MBL International, PM045MS).

Techniques: Staining, Two Tailed Test, Variant Assay

Journal: iScience

Article Title: The Drosophila Him gene is essential for adult muscle function and muscle stem cell maintenance

doi: 10.1016/j.isci.2026.114670

Figure Lengend Snippet: HimGFP design and expression in leg disc myoblasts and adult MuSCs (A) Schematic of the HimGFP “minigene” comprising 3.8 kb upstream genomic sequence, mGFP fused to the Him coding sequence via an -SSSS- linker, followed by the Him 3′ UTR. (B) Larval T2 leg discs labeled with anti-Mef2 and anti-GFP show co-expression of HimGFP and Mef2 (examples arrowed) in the associated myoblasts ( n = 4). Scale bars, 100 μm. (C) Sagittal thoracic section showing an adult MuSC (arrows) on the periphery of a DLM fiber (boxed area shown at higher magnification). The MuSC expresses HimGFP and canonical marker Zfh1, and stains with nuclear marker Hoechst ( n = 5). Scale bars, 100 μm. (D) Close-up of a sagittal thoracic section showing adult MuSCs (arrowed) on the periphery of a DLM fiber that express both HimGFP and Mef2 ( n = 3). Scale bars, 20 μm.

Article Snippet: Rabbit anti-Mef2 (1:1,000) , DSHB , Mef2.

Techniques: Expressing, Sequencing, Labeling, Marker

Journal: iScience

Article Title: The Drosophila Him gene is essential for adult muscle function and muscle stem cell maintenance

doi: 10.1016/j.isci.2026.114670

Figure Lengend Snippet: Him is required for normal jump muscle morphology and function, and genetically interacts with Mef2 (A–H) Transverse cryosections of adult jump muscle labeled with anti-βPSintegrin (green) and Hoechst nuclear stain (blue). (A) Control ( yw;;nos-Cas9 ) jump muscle section ( n = 12) consists of two organized rows of muscle fibers arranged around a mid-line (white arrow). The WT morphology is disrupted in 100% of (B) Him 0 mutant ( n = 12), (C) Him 52 mutant ( n = 7), and (D) Him 0 /Him 52 heteroallelic mutant flies ( n = 7). Him mutants have “internal fibers” that do not contact the outer edge of the jump muscle (orange arrow in B). Both the HimGFP minigene and the Him duplication restore WT morphology in Him 0 (E,G) and Him 52 (F,H) mutants ( n = 8–11 per genotype). Scale bars, 50 μm. (I) Bar graph showing mean internal muscle fiber counts ±SEM of the genotypes in (A–H). Him mutants have significantly more internal fibers than WT. Both Him GFP and the Him duplication restore counts to WT values. (Kruskal-Wallis followed by post hoc Dunn’s test: p < 0.001, control versus Him 52 ∗∗∗ p < 0.001, control versus Him 0 ∗∗∗ p < 0.001, control versus Him 52 / Him 0 ∗∗∗ p < 0.001, control versus Him 52 ;; Him GFP p = 0.5985 (n.s.), control versus Him 52 ; ; Him Dup p = 0.2506 (n.s.), control versus Him 0 ;; Him GFP p = 0.2784 (n.s.), control versus Him 0 ;; Him Dup n = 0.2506 (n.s.). (J–L) Transverse cryosections of (J) control ( yw;;nos-Cas9 n = 11), (K) Him 0 mutant ( n = 6) and (L) Him 0 ; Mef2 22.21 /+ flies ( n = 11). Scale bars, 50 μm. The Mef2 22.21 allele rescues the Him mutant morphology toward WT. (M) Bar graph showing mean internal muscle fiber counts ±SEM of the genotypes in (K–M). Him 0 ;Mef2 22.21 /+ flies have significantly fewer internal fibers than Him 0 mutants, but more internal fibers than control flies (Kruskal-Wallis followed by post hoc Dunn’s test: p < 0.001, control versus Him0 ∗∗∗ p < 0.001, control versus Him0;Mef2 22.21 /+ ∗∗ p = 0.0012, Him 0 versus Him0;Mef2 22.21 /+ ∗ p = 0.0363). (N) Bar graph showing mean horizontal jump ±SEM, in response to a looming stimulus ( n = 15–20 flies per genotype). Him 0 and Him 52 adults jump significantly less distance than yw;;nosCas9 controls. HimGFP rescues each Him mutant to WT phenotype. (ANOVA followed by post hoc Dunnett’s test: ANOVA p < 0.0001, control versus Him 0 ∗ p = 0.0151, control versus Him 0 ;; Him GFP/+ non-significant (n.s.) p = 0.7185, control versus Him 52 ∗∗∗ p = 0.0001, control versus Him 52 ; Him GFP/+ n.s. p > 0.9999).

Article Snippet: Rabbit anti-Mef2 (1:1,000) , DSHB , Mef2.

Techniques: Labeling, Staining, Control, Mutagenesis

Journal: iScience

Article Title: The Drosophila Him gene is essential for adult muscle function and muscle stem cell maintenance

doi: 10.1016/j.isci.2026.114670

Figure Lengend Snippet: Him mutants have a reduced wing disc-associated myoblast pool (A–C) L3 wing imaginal discs were stained for Mhc (green), and F-actin (red). Premature muscle differentiation is not observed in (A) control ( w 1118 ) discs ( n = 17), or (B) Him 0 mutants ( n = 20). (C) 1,151-Gal4-driven UAS-Mef2 was used as a positive control ( n = 7). Scale bars, 50 μm. (D–F) L3 larval wing imaginal discs were stained for Ebd1 (green), which labels all myoblasts, and phospho-histone H3 (PH3, red), which labels mitotic cells. Scale bars, 50 μm. The myoblast pool size (outlined) was reduced in (E) Him 0 mutants ( n = 8) compared to (D) controls ( n = 9), and restored (F) when mutants were combined with Dp(1;3)DC343 ( n = 6). (G) Quantification of myoblast number for each genotype. (ANOVA followed by post hoc Dunnett’s test: ANOVA p = 0.0067, control versus Him 0 ∗∗ p = 0.0064, control versus rescue n.s. p = 0.9728). (H) Quantification of proliferating myoblasts shows the fraction of phospho-histone H3 (PH3) expressing cells was reduced in Him 0 mutants compared to controls, and restored when mutants were combined with Dp(1;3)DC343 . (ANOVA followed by post hoc Dunnett’s test: ANOVA p = 0.0022, versus control versus Him 0 ∗∗ p = 0.002, control versus rescue n.s. p = 0.9064).

Article Snippet: Rabbit anti-Mef2 (1:1,000) , DSHB , Mef2.

Techniques: Staining, Control, Positive Control, Expressing